Application
Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-ILVBL (ER1911-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kDApplication
Fig2: Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (ER1911-43) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: Hcclm3(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti- ILVBL (ER1911-43) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Product Name | ILVBL |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetolactate synthase homolog Acetolactate synthase like protein Acetolactate synthase-like protein AHAS ILV2H 209L8 IlvB (bacterial acetolactate synthase) like IlvB like protein IlvB-like protein Ilvbl ILVBL_HUMAN. |
| Immunogen | KLH conjugated synthetic peptide derived from human ILVBL 301-400/632 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | HumanMouseRatDogPigHorse |
| Concentration | 1mg/ml |
| Alternative Names | Acetolactate synthase homolog Acetolactate synthase like protein Acetolactate synthase-like protein AHAS ILV2H 209L8 IlvB (bacterial acetolactate synthase) like IlvB like protein IlvB-like protein Ilvbl ILVBL_HUMAN. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | A1L0T0 |
|---|
Application
Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug Primary: Anti-ILVBL (ER1911-43) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68kD Observed band size: 68kD
Application
Fig2: Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-ILVBL antibody (ER1911-43) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: Hcclm3(Human) Cell Lysate at 30 ug MCF-7(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Primary: Anti- ILVBL (ER1911-43) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Positive Control | Expressed in all tissues tested, with highest expression in heart, pancreas and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: FC:3ug/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
