Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-IMPDH1 (ER1911-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug K562(Human) cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-IMPDH1 (ER1911-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (ER1911-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (ER1911-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | IMPDH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IMDH1_HUMAN IMP (inosine monophosphate) dehydrogenase 1 IMP dehydrogenase 1 IMPD 1 IMPD IMPD1 IMPDH 1 IMPDH I IMPDH-I Impdh1 Inosine 5' monophosphate dehydrogenase 1 Inosine monophosphate dehydrogenase 1 Inosine-5''-monophosphate dehydrogenase 1 Inosine-5'-monophosphate dehydrogenase 1 LCA11 RP10. |
| Product description | Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. |
| Immunogen | KLH conjugated synthetic peptide derived from human IMPDH1 301-400/599 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbitGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | IMDH1_HUMAN IMP (inosine monophosphate) dehydrogenase 1 IMP dehydrogenase 1 IMPD 1 IMPD IMPD1 IMPDH 1 IMPDH I IMPDH-I Impdh1 Inosine 5' monophosphate dehydrogenase 1 Inosine monophosphate dehydrogenase 1 Inosine-5''-monophosphate dehydrogenase 1 Inosine-5'-monophosphate dehydrogenase 1 LCA11 RP10. |
|---|---|
| Molecular Weight(MW) | 55 KDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P20839 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-IMPDH1 (ER1911-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig2: Sample: Jurkat(Human) Cell Lysate at 30 ug K562(Human) cell Lysate at 30 ug NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti-IMPDH1 (ER1911-45) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (ER1911-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IMPDH1) Polyclonal Antibody, Unconjugated (ER1911-45) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Positive Control | IMP type I is the main species in normal leukocytes and type II predominates over type I in the tumor. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
