Application
Fig1: Sample: A431(Human) Cell Lysate at 40 ug Primary: Anti- Inhibin beta B (ER1911-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kDApplication
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- Inhibin beta B (ER1911-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (ER1911-47) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | Inhibin beta B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC157939 Activin A Activin AB alpha polypeptide Activin AB Activin AB beta polypeptide Activin B Activin beta A chain Activin beta B chain ActivinA ActivinAB ActivinB EDF Erythroid differentiation protein follicle stimulating hormone releasing protein FRP FSH releasing factor FSH releasing protein INHBA INHBB Inhibin Inhibin beta 1 Inhibin beta 2 Inhibin beta A Inhibin beta A chain Inhibin beta A subunit Inhibin beta B Inhibin beta B chain Inhibin beta B subunit |
| Immunogen | KLH conjugated synthetic peptide derived from human Inhibin beta B 251-350/407 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | MGC157939 Activin A Activin AB alpha polypeptide Activin AB Activin AB beta polypeptide Activin B Activin beta A chain Activin beta B chain ActivinA ActivinAB ActivinB EDF Erythroid differentiation protein follicle stimulating hormone releasing protein FRP FSH releasing factor FSH releasing protein INHBA INHBB Inhibin Inhibin beta 1 Inhibin beta 2 Inhibin beta A Inhibin beta A chain Inhibin beta A subunit Inhibin beta B Inhibin beta B chain Inhibin beta B subunit |
|---|---|
| Molecular Weight(MW) | 13/45 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P09529 |
|---|
Application
Fig1: Sample: A431(Human) Cell Lysate at 40 ug Primary: Anti- Inhibin beta B (ER1911-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD
Application
Fig2: Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti- Inhibin beta B (ER1911-47) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Inhibin beta B) Polyclonal Antibody, Unconjugated (ER1911-47) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
