Application
Fig1: Blank control: U937(blue). Primary Antibody: Rabbit Anti-Integrin alpha 3 antibody(ER1911-51), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-51, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig2: Sample: Lung(Mouse) lysate at 30ug; Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 20/114 kD Observed band size: 20 kDApplication
Fig3: Sample: Intestinal(Mouse) lysate at 30ug; Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 20/114 kD Observed band size: 24 kD| Product Name | Integrin alpha 3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Integrin alpha 3 light chain AA407068 Antigen identified by monoclonal Integrin alpha-3 J143 CD49 antigen like family member C CD49 antigen-like family member C CD49c CD49c antigen FLJ34631 FLJ34704 FRP 2 FRP-2 FRP2 Galactoprotein B3 GAP B3 GAPB3 Integrin alpha 3 Integrin alpha-3 light chain Integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor) ITA3_HUMAN ITGA 3 Itga3 MSK18 VCA 2 VCA2 Very late activation protein 3 receptor alpha 3 subunit VL3A VLA 3 alpha chain VLA 3 subunit alpha VLA-3 subunit alpha VLA3a. |
| Product description | Integrin alpha-3 is a protein that in humans is encoded by the ITGA3 gene. ITGA3 is an integrin alpha subunit. Together with beta-1 subunit, it makes up half of the α3β1 integrin duplex that plays a role in neural migration and corticogenesis, acted upon by such factors as netrin-1 and reelin. ITGA3 encodes the integrin alpha 3 chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 3 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form an integrin that interacts with many extracellular matrix proteins. Expression of this gene may be correlated with breast cancer metastasis. |
| Immunogen | KLH conjugated synthetic peptide derived from human Integrin alpha 3 light chain 976-1025/1051 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatPig |
| Concentration | 1mg/ml |
| Alternative Names | Integrin alpha 3 light chain
AA407068
Antigen identified by monoclonal Integrin alpha-3 J143 CD49 antigen like family member C CD49 antigen-like family member C CD49c CD49c antigen FLJ34631 FLJ34704 FRP 2 FRP-2 FRP2 Galactoprotein B3 GAP B3 GAPB3 Integrin alpha 3 Integrin alpha-3 light chain Integrin alpha 3 (antigen CD49C alpha 3 subunit of VLA-3 receptor) ITA3_HUMAN ITGA 3 Itga3 MSK18 VCA 2 VCA2 Very late activation protein 3 receptor alpha 3 subunit VL3A VLA 3 alpha chain VLA 3 subunit alpha VLA-3 subunit alpha VLA3a. |
|---|---|
| Molecular Weight(MW) | 20/114 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | P26006 |
|---|
Application
Fig1: Blank control: U937(blue). Primary Antibody: Rabbit Anti-Integrin alpha 3 antibody(ER1911-51), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (ER1911-51, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: Lung(Mouse) lysate at 30ug; Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 20/114 kD Observed band size: 20 kD
Application
Fig3: Sample: Intestinal(Mouse) lysate at 30ug; Primary: Anti-Integrin alpha 3 (ER1911-51) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size: 20/114 kD Observed band size: 24 kD| Positive Control | Isoform 1 is widely expressed. Isoform 2 is expressed in brain and heart. In brain, both isoforms are exclusively expressed on vascular smooth muscle cells, whereas in heart isoform 1 is strongly expressed on vascular smooth muscle cells, isoform 2 is detected only on endothelial vein cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
