Application
Fig1: Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ITM2A/AF488 Conjugated antibody (ER1911-62-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.Application
Fig2: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-ITM2A (ER1911-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kDApplication
Fig3: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-ITM2A (ER1911-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD| Product Name | ITM2A |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BRICD2A BRICHOS domain containing 2A E25 protein E25A Integral membrane protein 2A ITM2A ITM2A_HUMAN Protein E25. |
| Immunogen | KLH conjugated synthetic peptide derived from human ITM2A 21-120/263 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatPigRabbit |
| Concentration | 1mg/ml |
| Alternative Names | BRICD2A BRICHOS domain containing 2A E25 protein E25A Integral membrane protein 2A ITM2A ITM2A_HUMAN Protein E25. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Membrane; Single-pass type II membraneprotein (Potential). |
| SwissProt ID | O43736 |
|---|
Application
Fig1: Blank control(blue): 293T Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ITM2A/AF488 Conjugated antibody (ER1911-62-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Application
Fig2: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-ITM2A (ER1911-62) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Application
Fig3: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-ITM2A (ER1911-62) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD| Application Notes | WB:1:500-2000: FC:1μg/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
