Application
Fig1: Sample: HepG2 cell (human) Lysate at 40 ug Primary: Anti- JIP1/MAPK8IP1 (ER1911-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 77 kD| Product Name | JIP1/MAPK8IP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C jun amino terminal kinase interacting protein 1 IB 1 IB1 Islet brain 1 JIP 1 JNK interacting protein 1 JNK MAP kinase scaffold protein 1 MAPK8IP 1 MAPK8IP1 Mitogen activated protein kinase 8 interacting protein 1 PRKM8 interacting protein PRKM8IP JIP1_HUMAN. |
| Product description | The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module and is required for JNK activation in response to excitotoxic stress. Cytoplasmic JIP1 causes inhibition of JNK-regulated activity by retaining JNK in the cytoplasm and inhibiting JNK phosphorylation of c-Jun. It may also participate in ApoER2-specific reelin signaling and directly, or indirectly, regulates GLUT2 gene expression and beta-cell function. It appears to have a role in cell signaling in mature and developing nerve terminals and may function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins. It functions as an anti-apoptotic protein whose level seems to influence the beta-cell death or survival response. |
| Immunogen | KLH conjugated synthetic peptide derived from human JIP1/MAPK8IP1 425-524/711 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | C jun amino terminal kinase interacting protein 1 IB 1 IB1 Islet brain 1 JIP 1 JNK interacting protein 1 JNK MAP kinase scaffold protein 1 MAPK8IP 1 MAPK8IP1 Mitogen activated protein kinase 8 interacting protein 1 PRKM8 interacting protein PRKM8IP JIP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 77 KDa |
| Cellular Localization | Cytoplasm (By similarity). Cytoplasm, perinuclear region (By similarity). Nucleus (By similarity). Endoplasmic reticulum membrane. Mitochondrion membrane. Note=Accumulates in cell surface projections. Under certain stress conditions, translocates to the perinuclear region of neurons. In insulin-secreting cells, detected in both the cytoplasm and nucleus |
| SwissProt ID | Q9UQF2 |
|---|
Application
Fig1: Sample: HepG2 cell (human) Lysate at 40 ug Primary: Anti- JIP1/MAPK8IP1 (ER1911-65) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 77 kD Observed band size: 77 kD| Positive Control | Highly expressed in brain. Expressed in neurons, localizing to neurite tips in differentiating cells. Also expressed in the pancreas, testis and prostate. Low levels in heart, ovary and small intestine. Decreased levels in pancreatic beta cells sensitize cells to IL-1-beta-induced apoptosis. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
