Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-JMJD6 (ER1911-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 44 kDApplication
Fig2: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-JMJD6 Polyclonal Antibody, Unconjugated(ER1911-66) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | JMJD6 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptotic cell clearance receptor Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 Histone arginine demethylase JMJD6 JmjC domain-containing protein 6 JMJD 6 JMJD6 JMJD6_HUMAN Jumonji domain containing 6 Jumonji domain-containing protein 6 KIAA0585 Lysyl-hydroxylase JMJD6 Peptide-lysine 5-dioxygenase JMJD6 Phosphatidylserine receptor Protein PTDSR PTDSR 1 PTDSR PTDSR1. |
| Product description | Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as a RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses. |
| Immunogen | KLH conjugated synthetic peptide derived from human JMJD6 261-360/403 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitZebra FishSheep |
| Concentration | 1mg/ml |
| Alternative Names | Apoptotic cell clearance receptor Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 Histone arginine demethylase JMJD6 JmjC domain-containing protein 6 JMJD 6 JMJD6 JMJD6_HUMAN Jumonji domain containing 6 Jumonji domain-containing protein 6 KIAA0585 Lysyl-hydroxylase JMJD6 Peptide-lysine 5-dioxygenase JMJD6 Phosphatidylserine receptor Protein PTDSR PTDSR 1 PTDSR PTDSR1. |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. Note=Mainly found throughout the nucleoplasm outside of regions containing heterochromatic DNA, with some localization in nucleolus. During mitosis, excluded from the nucleus and reappears in the telophase of the cell cycle. |
| SwissProt ID | Q6NYC1 |
|---|
Application
Fig1: Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-JMJD6 (ER1911-66) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 44 kD
Application
Fig2: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-JMJD6 Polyclonal Antibody, Unconjugated(ER1911-66) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Highly expressed in the heart, skeletal muscle and kidney. Expressed at moderate or low level in brain, placenta, lung, liver, pancreas, spleen, thymus, prostate, testis and ovary. Up-regulated in many patients with chronic pancreatitis. Expressed in nursing thymic epithelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
