Application
Fig1: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti- JMY (ER1911-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55/111 kD Observed band size: 111 kD| Product Name | JMY |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Jmy JMY protein JMY_HUMAN junction mediating and regulatory protein Junction-mediating and -regulatory protein MGC163496 WAS protein homology region 2 domain containing 1-like 3 WHDC1L3. |
| Product description | Acts both as a nuclear p53/TP53-cofactor and a cytoplasmic regulator of actin dynamics depending on conditions. In nucleus, acts as a cofactor that increases p53/TP53 response via its interaction with p300/EP300. Increases p53/TP53-dependent transcription and apoptosis, suggesting an important role in p53/TP53 stress response such as DNA damage. In cytoplasm, acts as a nucleation-promoting factor for both branched and unbranched actin filaments. Activates the Arp2/3 complex to induce branched actin filament networks. Also catalyzes actin polymerization in the absence of Arp2/3, creating unbranched filaments. Contributes to cell motility by controlling actin dynamics. May promote the rapid formation of a branched actin network by first nucleating new mother filaments and then activating Arp2/3 to branch off these filaments. The p53/TP53-cofactor and actin activator activities are regulated via its subcellular location (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human JMY 401-500/988 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Jmy JMY protein JMY_HUMAN junction mediating and regulatory protein Junction-mediating and -regulatory protein MGC163496 WAS protein homology region 2 domain containing 1-like 3 WHDC1L3. |
|---|---|
| Molecular Weight(MW) | 55, 111 KDa |
| Cellular Localization | Nucleus (By similarity). Cytoplasm, cytoskeleton. Note=Localizes to the nucleus in most cell types. Accumulates in nucleus under DNA damage conditions, increasing p53/TP53 transcription response and reducing its influence on cell motility (By similarity). In primary neutrophils, it colocalizes with actin filaments at the leading edge and is excluded from the nucleus. Localization correlates with motility, because it moves from the nucleus to the cytoplasmic compartment when cells are differentiated from nonmotile cells into highly motile neutrophil-like cells. |
| SwissProt ID | Q8N9B5 |
|---|
Application
Fig1: Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti- JMY (ER1911-67) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55/111 kD Observed band size: 111 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
