Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti-KATNA1 (ER1911-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:56 kD Observed band size: 56 kDApplication
Fig2: Sample: Fetal brain (Mouse) Lysate at 40 ug Primary: Anti-KATNA1 (ER1911-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:56 kD Observed band size: 56 kDApplication
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (ER1911-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (ER1911-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Product Name | KATNA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Katanin p60 A1 Katanin p60 ATPase containing subunit A1 Katanin p60 ATPase-containing subunit A1 Katanin p60 subunit A1 KATNA1 KTNA1_HUMAN p60 katanin. |
| Product description | Catalytic subunit of a complex which severs microtubules in an ATP-dependent manner. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human KATNA1/Katanin p60 A1 221-320/491 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Katanin p60 A1 Katanin p60 ATPase containing subunit A1 Katanin p60 ATPase-containing subunit A1 Katanin p60 subunit A1 KATNA1 KTNA1_HUMAN p60 katanin. |
|---|---|
| Molecular Weight(MW) | 56 KDa |
| Cellular Localization | Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton. Note=Predominantly cytoplasmic. Also localized to the interphase centrosome and the mitotic spindle poles. Enhanced recruitment to the mitotic spindle poles requires microtubules and interaction with KATNB1. |
| SwissProt ID | O75449 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug HepG2(Human) Cell Lysate at 30 ug Cerebral cortex (Mouse) Lysate at 40 ug Primary: Anti-KATNA1 (ER1911-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:56 kD Observed band size: 56 kD
Application
Fig2: Sample: Fetal brain (Mouse) Lysate at 40 ug Primary: Anti-KATNA1 (ER1911-68) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:56 kD Observed band size: 56 kD
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (ER1911-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KATNA1) Polyclonal Antibody, Unconjugated (ER1911-68) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
