Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- KCNH1 (ER1911-71) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kDApplication
Fig2: Blank control: Hela(blue). Primary Antibody:Rabbit Anti-KCNH1 antibody(ER1911-71), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-2442R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-2442R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | KCNH1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EAG1 EAG 1 EAG-1 KCNH 1 KCNH-1 h eag Kv10.1 MGC124420 MGC142269 EAG Ether a go go potassium channel 1 hEAG1 M eag MGC124419 Potassium voltage gated channel subfamily H (eag related), member 1 Voltage gated potassium channel subunit Kv10.1 KCNH1_HUMAN. |
| Product description | Pore-forming (alpha) subunit of voltage-gated non-inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts. |
| Immunogen | KLH conjugated synthetic peptide derived from human KCNH1 501-600/989 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | EAG1
EAG 1
EAG-1
KCNH 1
KCNH-1
h eag
Kv10.1
MGC124420
MGC142269
EAG
Ether a go go potassium channel 1
hEAG1
M eag
MGC124419
Potassium voltage gated channel subfamily H (eag related) member 1 Voltage gated potassium channel subunit Kv10.1 KCNH1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 111 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | O95259 |
|---|
Application
Fig1: Sample: Hippocampus (Mouse) Lysate at 40 ug Primary: Anti- KCNH1 (ER1911-71) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD
Application
Fig2: Blank control: Hela(blue). Primary Antibody:Rabbit Anti-KCNH1 antibody(ER1911-71), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min). Antibody (bs-2442R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody of bs-2442R at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Highly expressed in brain and in myoblasts at the onset of fusion, but not in other tissues. Detected in HeLa (cervical carcinoma), SH-SY5Y (neuroblastoma) and MCF-7 (epithelial tumor) cells, but not in normal epithelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
