Application
Fig1: Sample: Brain (Rat) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti-KCNN4 (ER1911-74) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 50 kD Observed band size: 48/42 kD| Product Name | KCNN4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hIKCa1 IK1 IKCa1 Intermediate conductance calcium activated potassium channel protein 4 Intermediate conductance calcium-activated potassium channel protein 4 KCa3.1 KCa4 KCNN 4 Kcnn4 KCNN4_HUMAN Potassium intermediate/small conductance calcium activated channel, subfamily N, member 4 Putative Gardos channel SK4 SKCa 4 SKCa4. |
| Product description | Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. |
| Immunogen | KLH conjugated synthetic peptide derived from human KCNN4 325-427/427 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | hIKCa1
IK1
IKCa1
Intermediate conductance calcium activated potassium channel protein 4
Intermediate conductance calcium-activated potassium channel protein 4
KCa3.1
KCa4
KCNN 4
Kcnn4
KCNN4_HUMAN
Potassium intermediate/small conductance calcium activated channel subfamily N member 4 Putative Gardos channel SK4 SKCa 4 SKCa4. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Membrane; Multi-pass membrane protein. |
| SwissProt ID | O15554 |
|---|
Application
Fig1: Sample: Brain (Rat) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti-KCNN4 (ER1911-74) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution Predicted band size: 50 kD Observed band size: 48/42 kD| Positive Control | Widely expressed in non-excitable tissues. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
