Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Keratan Sulfate (ER1911-80) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kDApplication
Fig2: Blank control(blue):RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Keratan Sulfate antibody(ER1911-80), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Product Name | Keratan Sulfate |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Carbohydrate chondroitin 6 keratan sulfotransferase 1 Carbohydrate chondroitin 6 keratan sulfotransferase Carbohydrate keratan sulfate Gal 6 sulfotransferase Carbohydrate keratan sulfate Gal 6 sulfotransferase 1 Carbohydrate sulfotransferase 1 CHST1 EC 2.8.2.21 Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1 GST 1 antibody Keratan sulfate Gal 6 sulfotransferase KS6ST KSGAL6ST KSST. |
| Product description | Catalyzes the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. |
| Immunogen | KLH conjugated synthetic peptide derived from human Keratan Sulfate 251-350/411 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Carbohydrate chondroitin 6 keratan sulfotransferase 1 Carbohydrate chondroitin 6 keratan sulfotransferase Carbohydrate keratan sulfate Gal 6 sulfotransferase Carbohydrate keratan sulfate Gal 6 sulfotransferase 1 Carbohydrate sulfotransferase 1 CHST1 EC 2.8.2.21 Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1 GST 1 antibody Keratan sulfate Gal 6 sulfotransferase KS6ST KSGAL6ST KSST. |
|---|---|
| Molecular Weight(MW) | 45 KDa |
| Cellular Localization | Golgi apparatus membrane. |
| SwissProt ID | O43916 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Keratan Sulfate (ER1911-80) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 45 kD
Application
Fig2: Blank control(blue):RSC96 cells(fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti-Keratan Sulfate antibody(ER1911-80), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.| Positive Control | Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
