Application
Fig1: Sample:Cerebellum (Mouse)Lysate at 40 ug Primary: Anti-KIAA0513(ER1911-84)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 47kD Observed band size: 47kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (ER1911-84) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (ER1911-84) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | KIAA0513 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | K0513_HUMAN KIAA0513 Uncharacterized protein KIAA0513. |
| Immunogen | KLH conjugated synthetic peptide derived from human KIAA0513 21-100/411 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | K0513_HUMAN KIAA0513 Uncharacterized protein KIAA0513. |
|---|---|
| Molecular Weight(MW) | 47 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | O60268 |
|---|
Application
Fig1: Sample:Cerebellum (Mouse)Lysate at 40 ug Primary: Anti-KIAA0513(ER1911-84)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 47kD Observed band size: 47kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (ER1911-84) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (KIAA0513) Polyclonal Antibody, Unconjugated (ER1911-84) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Widely expressed, highest levels in cerebellum, brain cortex, hippocampus, pons, putamen and amygdala. Highly expressed in neurons, but also present in glial cells. Slightly higher expression in the dorsolateral prefrontal cortex of schizophrenic patients compared to control individuals. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
