Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (ER1911-89) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (ER1911-89) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-Kinesin 5B (Bs-2385R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Product Name | Kinesin 5B |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kinesin 5B Kinesin5B Kinesin-5B Klf5 CKLF Krueppel-like factor 5 Intestinal-enriched krueppel-like factor Transcription factor BTEB2 Basic transcription element-binding protein 2 BTE-binding protein 2 Krueppel-like factor 5 KLF5 basic transcription element-binding protein 2 BTEB2 intestine enriched kr |
| Product description | This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. The encoded protein is a transcriptional activator that binds directly to a specific recognition motif in the promoters of target genes. This protein acts downstream of multiple different signaling pathways and is regulated by post-translational modification. It may participate in both promoting and suppressing cell proliferation. Expression of this gene may be changed in a variety of different cancers and in cardiovascular disease. Alternative splicing results in multiple transcript variants. |
| Immunogen | KLH conjugated synthetic peptide derived from human KLF5 61-160/457 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Kinesin 5B
Kinesin5B
Kinesin-5B Klf5 CKLF Krueppel-like factor 5 Intestinal-enriched krueppel-like factor Transcription factor BTEB2 Basic transcription element-binding protein 2 BTE-binding protein 2 Krueppel-like factor 5 KLF5 basic transcription element-binding protein 2 BTEB2 intestine enriched kr |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q13887 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (ER1911-89) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Kinesin 5B) Polyclonal Antibody, Unconjugated (ER1911-89) at 1:400 overnight at 4℃, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-Kinesin 5B (Bs-2385R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Positive Control | Expressed only in testis and placenta. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
