Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug Primary: Anti-Kir6.2 (ER1911-93) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 45 kDApplication
Fig2: Blank control: A549. Primary Antibody (green line): Rabbit Anti-Kir6.2 antibody (ER1911-93) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | Kir6.2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP sensitive inward rectifier potassium channel 11 Beta cell inward rectifier subunit mBIR BIR HHF 2 HHF2 IKATP Inward rectifier K(+) channel Inwardly rectifying potassium channel KIR6.2 IRK 11 IRK11 KCNJ11 Kir 6.2 MGC133230 PHHI Potassium channel, inwardly rectifying subfamily J member 11 Potassium inwardly rectifying channel J11 TNDM 3 TNDM3 IRK11_HUMAN. |
| Product description | This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation. |
| Immunogen | KLH conjugated synthetic peptide derived from human Kir62 301-390/390 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-2000: FC:3ug/test: IF:1:100-500: ICC:1:100-500: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ATP sensitive inward rectifier potassium channel 11
Beta cell inward rectifier subunit
mBIR
BIR
HHF 2
HHF2
IKATP
Inward rectifier K(+) channel Inwardly rectifying potassium channel KIR6.2
IRK 11
IRK11
KCNJ11
Kir 6.2
MGC133230
PHHI
Potassium channel inwardly rectifying subfamily J member 11 Potassium inwardly rectifying channel J11 TNDM 3 TNDM3 IRK11_HUMAN. |
|---|---|
| Molecular Weight(MW) | 43 KDa |
| Cellular Localization | Cell Membrane; Multi-pass membrane protein |
| SwissProt ID | Q14654 |
|---|
Application
Fig1: Sample: Pancreas (Mouse) Lysate at 40 ug Brain (Mouse) Lysate at 40 ug Primary: Anti-Kir6.2 (ER1911-93) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 45 kD
Application
Fig2: Blank control: A549. Primary Antibody (green line): Rabbit Anti-Kir6.2 antibody (ER1911-93) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:500-2000: FC:3ug/test: IF:1:100-500: ICC:1:100-500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
