Application
Fig1: Sample: Spinal cord (Mouse) Lysate at 40 ug Spinal cord (Rat) Lysate at 40 ug Primary:Anti-KiSS1 receptor (ER1911-94) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 59 kD| Product Name | KiSS1 receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AXOR 12 AXOR12 G protein coupled receptor 54 G-protein coupled receptor 54 G-protein coupled receptor OT7T175 GPCR 54 GPCR54 GPR 54 GPR54 HOT7T175 Hypogonadotropin 1 Hypogonadotropin-1 Hypogonadotropin1 KISS 1 receptor KISS 1R KiSS-1 receptor KiSS1R KiSS-1R KISS1 receptor KISS1R Kisspeptins receptor KISSR_HUMAN Metastin receptor OT7T175. |
| Product description | Receptor for metastin (kisspeptin-54 or kp-54), a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine-tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. |
| Immunogen | KLH conjugated synthetic peptide derived from human GPR54 121-200/398 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigSheepGuinea Pig |
| Concentration | 1mg/ml |
| Alternative Names | AXOR 12 AXOR12 G protein coupled receptor 54 G-protein coupled receptor 54 G-protein coupled receptor OT7T175 GPCR 54 GPCR54 GPR 54 GPR54 HOT7T175 Hypogonadotropin 1 Hypogonadotropin-1 Hypogonadotropin1 KISS 1 receptor KISS 1R KiSS-1 receptor KiSS1R KiSS-1R KISS1 receptor KISS1R Kisspeptins receptor KISSR_HUMAN Metastin receptor OT7T175. |
|---|---|
| Molecular Weight(MW) | 44 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | Q969F8 |
|---|
Application
Fig1: Sample: Spinal cord (Mouse) Lysate at 40 ug Spinal cord (Rat) Lysate at 40 ug Primary:Anti-KiSS1 receptor (ER1911-94) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 44 kD Observed band size: 59 kD| Positive Control | Most highly expressed in the pancreas, placenta and spinal cord, with lower-level of expression in peripheral blood leukocytes, kidney, lung, fetal liver, stomach, small intestine, testes, spleen, thymus, adrenal glands and lymph nodes. In the adult brain, expressed in the superior frontal gyrus, putamen, caudate nucleus, cingulate gyrus, nucleus accumbens, hippocampus, pons and amygdala, as well as the hypothalamus and pituitary. Expression levels are higher in early (7-9 weeks) than term placentas. Expression levels were increased in both early placentas and molar pregnancies and were reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation. Also found in the extravillous trophoblast suggesting endocrine/paracrine activation mechanism. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
