Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (ER1911-98) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.Application
Fig2: Sample: Lane1:Brain (Mouse) Lysate at 30 ug Lane2:HepG2 Cell Lysate at 30 ug Primary: Anti-KLF2 (ER1911-98) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :39 kD Observed band size :39 kDApplication
Fig3: Blank control: A431. Primary Antibody (green line): Rabbit Anti-KLF2 antibody (ER1911-98) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Product Name | KLF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kruppel like factor 2 Kruppel like factor Kruppel like factor LKLF Kruppel-like factor 2 (lung) Lung krueppel like factor Lung Kruppel like zinc finger transcription factor KLF 2 Klf2 KLF2_HUMAN Krueppel-like factor 2 Lklf Lung krueppel like factor Lung krueppel-like factor Lung Kruppel like zinc finger transcription factor. |
| Product description | Binds to the CACCC box in the beta-globin gene promoter and activates transcription . |
| Immunogen | KLH conjugated synthetic peptide derived from human KLF2 251-355/355 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PICC/IFFC |
| WB:1:100-500: IHC-P:1:400-800: FC:1:ug/Test: IF:1:100-500: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Kruppel like factor 2 Kruppel like factor Kruppel like factor LKLF Kruppel-like factor 2 (lung) Lung krueppel like factor Lung Kruppel like zinc finger transcription factor KLF 2 Klf2 KLF2_HUMAN Krueppel-like factor 2 Lklf Lung krueppel like factor Lung krueppel-like factor Lung Kruppel like zinc finger transcription factor. |
|---|---|
| Molecular Weight(MW) | 39 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9Y5W3 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (KLF2) Polyclonal Antibody, Unconjugated (ER1911-98) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Application
Fig2: Sample: Lane1:Brain (Mouse) Lysate at 30 ug Lane2:HepG2 Cell Lysate at 30 ug Primary: Anti-KLF2 (ER1911-98) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000 dilution; Predicted band size :39 kD Observed band size :39 kD
Application
Fig3: Blank control: A431. Primary Antibody (green line): Rabbit Anti-KLF2 antibody (ER1911-98) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.| Application Notes | WB:1:100-500: IHC-P:1:400-800: FC:1:ug/Test: IF:1:100-500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
