Application
Fig1: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-KLHL3 (ER1912-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kDApplication
Fig2: Sample: HL-60 Cell (Human) Lysate at 40 ug Primary: Anti-KLHL3 (ER1912-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kD| Product Name | KLHL3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KLHL 3 FLJ40871 kelch (Drosophila) like 3 kelch like 3 (Drosophila) kelch like 3 Kelch like protein 3 KIAA1129 MGC44594 KLHL3_HUMAN. |
| Product description | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a regulator of ion transport in the distal nephron. The BCR(KLHL3) complex may act by mediating ubiquitination of SLC12A3/NCC, thereby regulating SLC12A3/NCC subcellular location at the cell membrane. |
| Immunogen | KLH conjugated synthetic peptide derived from human KLHL3 51-160/587 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | KLHL 3 FLJ40871 kelch (Drosophila) like 3 kelch like 3 (Drosophila) kelch like 3 Kelch like protein 3 KIAA1129 MGC44594 KLHL3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 65 KDa |
| Cellular Localization | Cytoplasm, cytoskeleton. Cytoplasm, cytosol. |
| SwissProt ID | Q9UH77 |
|---|
Application
Fig1: Sample: A549 Cell (Human) Lysate at 40 ug Primary: Anti-KLHL3 (ER1912-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kD
Application
Fig2: Sample: HL-60 Cell (Human) Lysate at 40 ug Primary: Anti-KLHL3 (ER1912-06) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 60 kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
