Application
Fig1: Tissue/cell: Human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT3C Polyclonal Antibody, Unconjugated(ER1912-08) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: heart (mouse) Lysate at 40 ug Primary: Anti-KMT3C(ER1912-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Product Name | KMT3C |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone methyltransferase SMYD2 HSKM B HSKM-B HSKMB KMT3C Lysine N-methyltransferase 3C MGC119305 N lysine methyltransferase SMYD2 N-lysine methyltransferase SMYD2 SET and MYND domain containing 2 SET and MYND domain containing protein 2 SET and MYND domain-containing protein 2 Smyd2 SMYD2_HUMAN Zinc finger MYND domain containing 14 ZMYND14. |
| Product description | Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. |
| Immunogen | KLH conjugated synthetic peptide derived from human KMT3C 351-433/433 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | Histone methyltransferase SMYD2 HSKM B HSKM-B HSKMB KMT3C Lysine N-methyltransferase 3C MGC119305 N lysine methyltransferase SMYD2 N-lysine methyltransferase SMYD2 SET and MYND domain containing 2 SET and MYND domain containing protein 2 SET and MYND domain-containing protein 2 Smyd2 SMYD2_HUMAN Zinc finger MYND domain containing 14 ZMYND14. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Cytoplasm; cytosol. Nucleus. |
| SwissProt ID | Q9NRG4 |
|---|
Application
Fig1: Tissue/cell: Human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-KMT3C Polyclonal Antibody, Unconjugated(ER1912-08) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: heart (mouse) Lysate at 40 ug Primary: Anti-KMT3C(ER1912-08) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
