Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- KMT4 (ER1912-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 185 kD Observed band size: 170 kD| Product Name | KMT4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Disrupter of telomere silencing protein 1 DOT 1 DOT1 DOT1 like DOT1 like histone H3 methyltransferase (S. cerevisiae) DOT1 like histone H3 methyltransferase DOT1 like protein DOT1, S. cerevisiae, homolog of DOT1L EC 2.1.1.43 H3 K79 HMTase Histone H3 K79 methyltransferase Histone H3 methyltransferase Histone H3 methyltransferase DOT1-like Histone lysine N methyltransferase H3 lysine 79 specific Histone methyltransferase DOT1L KIAA1814 DOT1L_HUMAN KMT4 Lysine N methyltransferase 4. |
| Product description | The KMT4 / Dot1 (disruptor of telomeric silencing) family are a class of histone methyltransferases that play an indirect role in chromatin silencing by targeting a conserved lysine residue in the nucleosome core. Biochemical studies suggest that recombinant Dot1 proteins are active on recombinant nucleosomes, free of any modifications. |
| Immunogen | KLH conjugated synthetic peptide derived from human KMT4 51-150/1739 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatCowSheepAfrican Green Monkey |
| Concentration | 1mg/ml |
| Alternative Names | Disrupter of telomere silencing protein 1
DOT 1
DOT1
DOT1 like
DOT1 like histone H3 methyltransferase (S. cerevisiae)
DOT1 like histone H3 methyltransferase
DOT1 like protein
DOT1 S. cerevisiae homolog of DOT1L EC 2.1.1.43 H3 K79 HMTase Histone H3 K79 methyltransferase Histone H3 methyltransferase Histone H3 methyltransferase DOT1-like Histone lysine N methyltransferase H3 lysine 79 specific Histone methyltransferase DOT1L KIAA1814 DOT1L_HUMAN KMT4 Lysine N methyltransferase 4. |
|---|---|
| Molecular Weight(MW) | 185 KDa |
| Cellular Localization | Nuclear |
| SwissProt ID | Q8TEK3 |
|---|
Application
Fig1: Sample: Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- KMT4 (ER1912-09) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 185 kD Observed band size: 170 kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
