Application
Fig1: Sample: Breast milk (Human) Lysate at 40 ug Primary: Anti-Lactoferrin (ER1912-16) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 76 kDApplication
Fig2: Sample: Lactoferrin protein (Human) Lysate at 100 ng Primary: Anti-Lactoferrin (ER1912-16) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 85 kDApplication
Fig3: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(ER1912-16) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).| Product Name | Lactoferrin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CKRX GIG12 Growth inhibiting protein 12 HLF2 Homo sapiens chemokine receptor X Kaliocin 1 Lactoferrin Lactoferroxin A Lactoferroxin B Lactoferroxin C Lactoferroxin-C Lactotransferrin LF LTF Neutrophil lactoferrin Talalactoferrin alfa Talalactoferrin TRFL_HUMAN. |
| Product description | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. ;Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. ;Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. ;The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity. ;Isoform DeltaLf: transcription factor with antiproliferative properties and inducing cell cycle arrest. Binds to DeltaLf response element found in the SKP1, BAX, DCPS, and SELH promoters. |
| Immunogen | KLH conjugated synthetic peptide derived from human Lactoferrin 611-710/710 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:0.2ug/test: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | CKRX GIG12 Growth inhibiting protein 12 HLF2 Homo sapiens chemokine receptor X Kaliocin 1 Lactoferrin Lactoferroxin A Lactoferroxin B Lactoferroxin C Lactoferroxin-C Lactotransferrin LF LTF Neutrophil lactoferrin Talalactoferrin alfa Talalactoferrin TRFL_HUMAN. |
|---|---|
| Molecular Weight(MW) | 76 KDa |
| Cellular Localization | Isoform 1: Secreted. Isoform DeltaLf: Cytoplasm. Nucleus. |
| SwissProt ID | P02788 |
|---|
Application
Fig1: Sample: Breast milk (Human) Lysate at 40 ug Primary: Anti-Lactoferrin (ER1912-16) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 76 kD
Application
Fig2: Sample: Lactoferrin protein (Human) Lysate at 100 ng Primary: Anti-Lactoferrin (ER1912-16) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 76 kD Observed band size: 85 kD
Application
Fig3: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Lactoferrin Antibody(ER1912-16) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).| Application Notes | WB:1:500-2000: FC:0.2ug/test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
