Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-LAG-3 (ER1912-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kDApplication
Fig2: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-LAG-3 (ER1912-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Product Name | LAG-3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lymphocyte activation gene 3 CD223 FDC protein LAG 3 LAG3 Lymphocyte activation gene 3 protein LAG3_HUMAN. |
| Product description | Involved in lymphocyte activation. Binds to HLA class-II antigens (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human LAG-3 201-300/525 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Lymphocyte activation gene 3 CD223 FDC protein LAG 3 LAG3 Lymphocyte activation gene 3 protein LAG3_HUMAN. |
|---|---|
| Molecular Weight(MW) | 58 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | Q5BK54 |
|---|
Application
Fig1: Sample: 293T Cell (Human) Lysate at 40 ug Primary: Anti-LAG-3 (ER1912-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD
Application
Fig2: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-LAG-3 (ER1912-17) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 58 kD Observed band size: 58 kD| Positive Control | On cell surface of activated NK and T-lymphocytes. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
