Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Lamin B Receptor (ER1912-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kDApplication
Fig2: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Lamin B Receptor antibody (bs-5801R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Lamin B Receptor (ER1912-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Product Name | Lamin B Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LBR_HUMAN LMN2R PHA DHCR 14B DHCR14B antibody Integral nuclear envelope inner membrane protein Lamin-B receptor LBR LMN 2R LMN2R MGC9041 PHA PRO0650 DHCR14B MGC9041. |
| Product description | Anchors the lamina and the heterochromatin to the inner nuclear membrane. |
| Immunogen | KLH conjugated synthetic peptide derived from human Lamin B Receptor 1-100/615 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1ug/Test: |
|
| Species Reactivity | HumanMouseRatCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | LBR_HUMAN LMN2R PHA DHCR 14B DHCR14B antibody Integral nuclear envelope inner membrane protein Lamin-B receptor LBR LMN 2R LMN2R MGC9041 PHA PRO0650 DHCR14B MGC9041. |
|---|---|
| Molecular Weight(MW) | 68 KDa |
| Cellular Localization | Nucleus inner membrane; Multi-pass membrane protein. |
| SwissProt ID | Q14739 |
|---|
Application
Fig1: Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Lamin B Receptor (ER1912-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD
Application
Fig2: Blank control (Black line):Molt4 (Black). Primary Antibody (green line): Rabbit Anti-Lamin B Receptor antibody (bs-5801R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig3: Sample: Hela(Human) Cell Lysate at 30 ug Primary: Anti-Lamin B Receptor (ER1912-18) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 68 kD Observed band size: 68 kD| Application Notes | WB:1:500-2000: FC:1ug/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
