Application
Fig1: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LGALS3BP Polyclonal Antibody, Unconjugated(ER1912-28) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti-LGALS3BP (ER1912-28) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD| Product Name | LGALS3BP |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mac 2 binding protein MAC2BP Tumor associated antigen 90K 90K 90K MAC 2 BP Basement membrane autoantigen p105 Galectin 3 binding protein L3 antigen Lectin galactoside binding soluble 3 binding protein M2BP Mac 2 BP Serum protein 90K LG3BP_HUMAN. |
| Product description | The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. |
| Immunogen | KLH conjugated synthetic peptide derived from human LGALS3BP 301-400/585 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Mac 2 binding protein
MAC2BP
Tumor associated antigen 90K 90K 90K MAC 2 BP Basement membrane autoantigen p105 Galectin 3 binding protein L3 antigen Lectin galactoside binding soluble 3 binding protein M2BP Mac 2 BP Serum protein 90K LG3BP_HUMAN. |
|---|---|
| Molecular Weight(MW) | 63 KDa |
| Cellular Localization | Secreted. Secreted, extracellular space, extracellular matrix. |
| SwissProt ID | Q08380 |
|---|
Application
Fig1: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LGALS3BP Polyclonal Antibody, Unconjugated(ER1912-28) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: Heart (Mouse) Lysate at 40 ug Heart (Rat) Lysate at 40 ug Primary: Anti-LGALS3BP (ER1912-28) at 1/2000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kD Observed band size: 63 kD| Positive Control | Ubiquitous. Detected in body fluids such as semen, milk, serum, tears, saliva and urine. Expressed by keratinocytes and fibroblasts. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
