Application
Fig1: Sample:
Large intestine(mouse) Lysate at 40 ug
U87mg cell(human) Lysate at 30 ug
Primary: Anti- LIF (ER1912-32) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 20kD
Observed band size: 30/50 kD