Application
Fig1: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-LIN9 (ER1912-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kDApplication
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LIN9 Polyclonal Antibody, Unconjugated(ER1912-37) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | LIN9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BARA Beta subunit-associated regulator of apoptosis Beta-subunit associated regulator of apoptosis hLin-9 huLin-9 lin-9 lin9 LIN9_HUMAN pRB-associated protein Protein lin-9 homolog TGS TUDOR gene similar protein Type I interferon receptor beta chain-associated protein. |
| Product description | Acts as a tumor suppressor. Inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. Plays a role in the expression of genes required for the G1/S transition. |
| Immunogen | KLH conjugated synthetic peptide derived from human LIN9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseCynomolgus Monkey |
| Concentration | 1mg/ml |
| Alternative Names | BARA Beta subunit-associated regulator of apoptosis Beta-subunit associated regulator of apoptosis hLin-9 huLin-9 lin-9 lin9 LIN9_HUMAN pRB-associated protein Protein lin-9 homolog TGS TUDOR gene similar protein Type I interferon receptor beta chain-associated protein. |
|---|---|
| Molecular Weight(MW) | 62 KDa |
| Cellular Localization | Nucleus > nucleoplasm. Found in perinucleolar structures. Associated with chromatin. |
| SwissProt ID | Q5TKA1 |
|---|
Application
Fig1: Sample: Bone (Mouse) Lysate at 40 ug Primary: Anti-LIN9 (ER1912-37) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 62 kD Observed band size: 62 kD
Application
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-LIN9 Polyclonal Antibody, Unconjugated(ER1912-37) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Expressed in thymus and testis. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
