Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Liprin alpha 2 (ER1912-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 143 kD Observed band size: 143 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIPA2) Polyclonal Antibody, Unconjugated (ER1912-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIPA2) Polyclonal Antibody, Unconjugated (ER1912-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | Liprin alpha 2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LIPA2_HUMAN Liprin-alpha-2 PPFIA2 Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 2 Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2 Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 2 PTPRF interacting protein alpha 2 PTPRF-interacting protein alpha-2. |
| Product description | Alters PTPRF cellular localization and induces PTPRF clustering. May regulate the disassembly of focal adhesions. May localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. |
| Immunogen | KLH conjugated synthetic peptide derived from human Liprin alpha 2 531-630/1257 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenPigHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | LIPA2_HUMAN
Liprin-alpha-2
PPFIA2
Protein tyrosine phosphatase receptor type f polypeptide interacting protein alpha 2
Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-2
Protein tyrosine phosphatase receptor type f polypeptide (PTPRF) interacting protein (liprin) alpha 2 PTPRF interacting protein alpha 2 PTPRF-interacting protein alpha-2. |
|---|---|
| Molecular Weight(MW) | 143 KDa |
| Cellular Localization | Cytoplasm. Cell surface. Colocalizes with PTPRF at the cell surface. |
| SwissProt ID | O75334 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-Liprin alpha 2 (ER1912-38) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 143 kD Observed band size: 143 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIPA2) Polyclonal Antibody, Unconjugated (ER1912-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIPA2) Polyclonal Antibody, Unconjugated (ER1912-38) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Expressed only in brain. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
