Application
Fig1: Sample:Lymph node (Mouse) Lysate at 40 ug Primary: Anti-Livin (ER1912-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD| Product Name | Livin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Baculoviral IAP repeat containing 7 Baculoviral IAP repeat containing protein 7 BIRC 7 BIRC7 BIRC7_HUMAN KIAP Kidney inhibitor of apoptosis protein LIVIN Livin inhibitor of apotosis Melanoma inhibitor of apoptosis protein ML IAP MLIAP RING finger protein 50 RNF 50 RNF50 Inhibitors of apoptosis proterins Livin. |
| Product description | Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits caspase-3 and proteolytic activation of pro-caspase-9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human Livin 101-200/298 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogCow |
| Concentration | 1mg/ml |
| Alternative Names | Baculoviral IAP repeat containing 7 Baculoviral IAP repeat containing protein 7 BIRC 7 BIRC7 BIRC7_HUMAN KIAP Kidney inhibitor of apoptosis protein LIVIN Livin inhibitor of apotosis Melanoma inhibitor of apoptosis protein ML IAP MLIAP RING finger protein 50 RNF 50 RNF50 Inhibitors of apoptosis proterins Livin. |
|---|---|
| Molecular Weight(MW) | 33 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Nuclear, and in a filamentous pattern throughout the cytoplasm. |
| SwissProt ID | Q96CA5 |
|---|
Application
Fig1: Sample:Lymph node (Mouse) Lysate at 40 ug Primary: Anti-Livin (ER1912-40) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD| Positive Control | Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
