Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- LOX1 (ER1912-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kD Observed band size: 50 kDApplication
Fig2: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-LOX 1 (ER1912-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kD Observed band size: 50 kDApplication
Fig3: Positive control: A549 cells Concebtration: 1μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.Application
Fig4: Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti- LOX 1 antibody(ER1912-42), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1912-42, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (ER1912-42) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig6: Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (ER1912-42) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | LOX 1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lectin-type oxidized LDL receptor 1 OLR1 lectin-like low density lipoprotein receptor-1 Oxidized low-density lipoprotein receptor 1 Ox-LDL receptor 1 Lectin-like oxidized LDL receptor 1 Lectin-like oxLDL receptor 1 LOX-1 BLOX1 Contains: Oxidized low-density lipoprotein receptor 1, soluble form A Oxidized low-density lipoprotein receptor 1, soluble form B OLR1_HUMAN. |
| Product description | Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. |
| Immunogen | KLH conjugated synthetic peptide derived from rabbit LOX-1 201-273/273 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
|
| Species Reactivity | HumanMouseRatCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Lectin-type oxidized LDL receptor 1
OLR1
lectin-like low density lipoprotein receptor-1
Oxidized low-density lipoprotein receptor 1
Ox-LDL receptor 1
Lectin-like oxidized LDL receptor 1
Lectin-like oxLDL receptor 1
LOX-1
BLOX1
Contains: Oxidized low-density lipoprotein receptor 1 soluble form A Oxidized low-density lipoprotein receptor 1 soluble form B OLR1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 31/50 KDa |
| Cellular Localization | Cell membrane; Single-pass type II membrane protein. Secreted. Note=A secreted form also exists. |
| SwissProt ID | Q9XTA8 |
|---|
Application
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug Primary: Anti- LOX1 (ER1912-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kD Observed band size: 50 kD
Application
Fig2: Sample: 293T(Human) Cell Lysate at 30 ug Primary: Anti-LOX 1 (ER1912-42) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 31/50 kD Observed band size: 50 kD
Application
Fig3: Positive control: A549 cells Concebtration: 1μg/10^6 cells Incubation conditions: Avoid light , 30 minutes on the ice.
Application
Fig4: Blank control: RSC96 (blue). Primary Antibody:Rabbit Anti- LOX 1 antibody(ER1912-42), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (ER1912-42, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Application
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (ER1912-42) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig6: Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (ER1912-42) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Highly expressed in endothelial cells, aortic intima and lung. Expressed at low level in other tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1μg /test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
