Application
Fig1: Sample:Heart (Mouse)Lysate at 40 ug Primary: Anti-LPA1(ER1912-44)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 44 kDApplication
Fig2: Sample:Kidney(Mouse)Lysate at 40 ug Primary: Anti-LPA1(ER1912-44)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 44 kD| Product Name | LPA1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDG 2 Endothelial Cell Differentiation Gene-2 Endothelial differentiation gene 2 Endothelial Differentiation Lysophosphatidic Acid G Protein Coupled Receptor 2 G10 protein homolog GPCR 26 GPCR26 Gpcr91 Kdt2 LPA 1 LPA receptor 1 LPA receptor EDG2 LPA1 LPAR1 Lysophosphatidic acid receptor 1 Lysophosphatidic acid receptor EDG2 MGC105279 MGC29102 LPAR1_HUMAN. |
| Product description | Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Stimulates phospholipase C (PLC) activity in a manner that is dependent on RALA activation. |
| Immunogen | KLH conjugated synthetic peptide derived from human EDG2/LPA1 281-364/364 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseSheep |
| Concentration | 1mg/ml |
| Alternative Names | EDG 2 Endothelial Cell Differentiation Gene-2 Endothelial differentiation gene 2 Endothelial Differentiation Lysophosphatidic Acid G Protein Coupled Receptor 2 G10 protein homolog GPCR 26 GPCR26 Gpcr91 Kdt2 LPA 1 LPA receptor 1 LPA receptor EDG2 LPA1 LPAR1 Lysophosphatidic acid receptor 1 Lysophosphatidic acid receptor EDG2 MGC105279 MGC29102 LPAR1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 41 KDa |
| Cellular Localization | Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA co-localizes with RALA in the endocytic vesicles. |
| SwissProt ID | Q92633 |
|---|
Application
Fig1: Sample:Heart (Mouse)Lysate at 40 ug Primary: Anti-LPA1(ER1912-44)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 44 kD
Application
Fig2: Sample:Kidney(Mouse)Lysate at 40 ug Primary: Anti-LPA1(ER1912-44)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 44 kD| Positive Control | Expressed in many adult organs, including brain, heart, colon, small intestine, placenta, prostate, ovary, pancreas, testes, spleen, skeletal muscle, and kidney. Little or no expression in liver, lung, thymus, or peripheral blood leukocytes. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
