Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Lubricin) Polyclonal Antibody, Unconjugated (ER1912-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Lubricin (ER1912-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 152 kD Observed band size: 152 kD| Product Name | Lubricin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Superficial zone proteoglycan articular superficial zone protein bG174L6.2 CACP camptodactyly arthropathy coxa vara pericarditis syndrome gene FLJ32635 HAPO Jacobs camptodactyly-arthropathy-pericarditis syndrome gene JCAP megakaryocyte stimulating factor MSF PRG 4 PRG4 proteoglycan 4 Proteoglycan4 Superficial zone proteoglycan SZP PRG4_HUMAN. |
| Product description | Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. ;Isoform F plays a role as a growth factor acting on the primitive cells of both hematopoietic and endothelial cell lineages. soluble molecule that acts as a carrier for insoluble surface-active phospholipid (SAPL). Depletion of lubricin function has been associated with camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP), an arthritic-like autosomal recessive disorder. |
| Immunogen | KLH conjugated synthetic peptide derived from human Lubricin/SZP 1151-1250/1404 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | Superficial zone proteoglycan articular superficial zone protein bG174L6.2 CACP camptodactyly arthropathy coxa vara pericarditis syndrome gene FLJ32635 HAPO Jacobs camptodactyly-arthropathy-pericarditis syndrome gene JCAP megakaryocyte stimulating factor MSF PRG 4 PRG4 proteoglycan 4 Proteoglycan4 Superficial zone proteoglycan SZP PRG4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 152 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | Q92954 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Lubricin) Polyclonal Antibody, Unconjugated (ER1912-53) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-Lubricin (ER1912-53) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 152 kD Observed band size: 152 kD| Positive Control | Highly expressed in synovial tissue, cartilage and liver and weakly in heart and lung. Isoform B is expressed in kidney, lung, liver, heart and brain. Isoform C and isoform D are widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
