Application
Fig1: Sample: Lymph node (Mouse) Lysate at 30 ug Spleen (Mouse) Lysate at 30 ug Primary: Anti- MAdCAM-1 (ER1912-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD| Product Name | MAdCAM-1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mucosal addressin cellular adhesion molecule-1 Addressin mucosal hMAdCAM 1 MACAM1 Mucosal addressin cell adhesion molecule 1 Mucosal vascular addressin cell adhesion molecule 1 MADCA_RAT. |
| Product description | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from rat MAdCAM-1 151-250/394 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Mucosal addressin cellular adhesion molecule-1 Addressin mucosal hMAdCAM 1 MACAM1 Mucosal addressin cell adhesion molecule 1 Mucosal vascular addressin cell adhesion molecule 1 MADCA_RAT. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Membrane; Single-pass type I membrane protein. |
| SwissProt ID | O70540 |
|---|
Application
Fig1: Sample: Lymph node (Mouse) Lysate at 30 ug Spleen (Mouse) Lysate at 30 ug Primary: Anti- MAdCAM-1 (ER1912-60) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 50 kD| Positive Control | Detected in Peyer patches and mesenteric lymph nodes but not in spleen. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
