Application
Fig1: Sample: Lung(Mouse) Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Large intestine(Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Smad7) Polyclonal Antibody, Unconjugated (ER1912-61) at 1:600 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.Application
Fig3: Sample:Lymph node (Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kDApplication
Fig4: Sample:Spleen(Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kDApplication
Fig5: Sample:Stomach (Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD| Product Name | MADH7/Smad7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hSMAD 7 hSMAD7 MAD (mothers against decapentaplegic Drosophila) homolog 7 MAD Mad homolog 7 MAD mothers against decapentaplegic homolog 7 MADH 7 MADH 8 MADH8 Mothers Against Decapentaplegic Drosophila Homolog of 7 Mothers against decapentaplegic homolog 7 Mothers against decapentaplegic homolog 8 Mothers against DPP homolog 7 Mothers against DPP homolog 8 SMA- AND MAD-RELATED PROTEIN 7 SMAD 7 SMAD SMAD family member 7 SMAD, mothers against DPP homolog 7 (Drosophila) SMAD, mothers against DPP homolog 7 Smad7 SMAD7_HUMAN. |
| Product description | Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. |
| Immunogen | KLH conjugated synthetic peptide derived from human Smad7 1-100/426 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCow |
| Concentration | 1mg/ml |
| Alternative Names | hSMAD 7
hSMAD7
MAD (mothers against decapentaplegic Drosophila) homolog 7
MAD
Mad homolog 7
MAD mothers against decapentaplegic homolog 7
MADH 7
MADH 8
MADH8
Mothers Against Decapentaplegic Drosophila Homolog of 7
Mothers against decapentaplegic homolog 7
Mothers against decapentaplegic homolog 8
Mothers against DPP homolog 7
Mothers against DPP homolog 8
SMA- AND MAD-RELATED PROTEIN 7
SMAD 7
SMAD
SMAD family member 7
SMAD mothers against DPP homolog 7 (Drosophila) SMAD mothers against DPP homolog 7 Smad7 SMAD7_HUMAN. |
|---|---|
| Molecular Weight(MW) | 46 KDa |
| Cellular Localization | Nucleus. Cytoplasm. Note=Interaction with NEDD4L or RNF111 or induces translocation from the nucleus to the cytoplasm. TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta. |
| SwissProt ID | O15105 |
|---|
Application
Fig1: Sample: Lung(Mouse) Lysate at 30 ug Placenta(Mouse) Lysate at 30 ug Large intestine(Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Smad7) Polyclonal Antibody, Unconjugated (ER1912-61) at 1:600 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Application
Fig3: Sample:Lymph node (Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD
Application
Fig4: Sample:Spleen(Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD
Application
Fig5: Sample:Stomach (Mouse) Lysate at 30 ug Primary: Anti- MADH7/Smad7 (ER1912-61) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 50 kD| Positive Control | Ubiquitous with higher expression in the lung and vascular endothelium. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
