Application
Fig1: Sample:U251 Cell Lysate at 40 ug Primary: Anti-MAG (ER1912-62) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:67 kD Observed band size:63 kDApplication
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MAG Polyclonal Antibody, Unconjugated(ER1912-62) 1:600, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | MAG |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Myelin associated glycoprotein L-MAG brain-specific neuronal polypeptide 1B236 Myelin-associated glycoprotein Sialic acid-binding Ig-like lectin 4a Siglec-4a Brain neuron cytoplasmic protein 3 MAG_HUMAN GMA MAG S MAG Siglec 4a SIGLEC4A. |
| Product description | Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. |
| Immunogen | KLH conjugated synthetic peptide derived from human MAG 551-626/626 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Myelin associated glycoprotein L-MAG brain-specific neuronal polypeptide 1B236 Myelin-associated glycoprotein Sialic acid-binding Ig-like lectin 4a Siglec-4a Brain neuron cytoplasmic protein 3 MAG_HUMAN GMA MAG S MAG Siglec 4a SIGLEC4A. |
|---|---|
| Molecular Weight(MW) | 67 KDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P20916 |
|---|
Application
Fig1: Sample:U251 Cell Lysate at 40 ug Primary: Anti-MAG (ER1912-62) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size:67 kD Observed band size:63 kD
Application
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MAG Polyclonal Antibody, Unconjugated(ER1912-62) 1:600, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
