Application
Fig1: Sample: Cerebrum(Mouse) Cell Lysate at 40 ug Primary: Anti-MAGI3(ER1912-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 166kD Observed band size: 180kD| Product Name | MAGI3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ730K3.2 MAGI-3 magi3 MAGI3_HUMAN Membrane Associated Guanylate kinase Related 3 Membrane-associated guanylate kinase Membrane-associated guanylate kinase inverted 3 RP4-730K3.1 SLIPR WW and PDZ domain-containing protein 3. |
| Product description | Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. |
| Immunogen | KLH conjugated synthetic peptide derived from human MAGI3 181-280/1506 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | dJ730K3.2 MAGI-3 magi3 MAGI3_HUMAN Membrane Associated Guanylate kinase Related 3 Membrane-associated guanylate kinase Membrane-associated guanylate kinase inverted 3 RP4-730K3.1 SLIPR WW and PDZ domain-containing protein 3. |
|---|---|
| Molecular Weight(MW) | 166 KDa |
| Cellular Localization | Cell membrane. Cell junction > tight junction. Nucleus. Concentrates in specific sites at the plasma membrane and in the nucleus. In epithelial cells, it localizes at tight junctions. |
| SwissProt ID | Q5TCQ9 |
|---|
Application
Fig1: Sample: Cerebrum(Mouse) Cell Lysate at 40 ug Primary: Anti-MAGI3(ER1912-66) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 166kD Observed band size: 180kD| Positive Control | Widely expressed. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
