Application
Fig1: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-MAP3K13/LZK (ER1912-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (ER1912-68) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Product Name | MAP3K13/LZK |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EC 2.7.11.25 Leucine zipper bearing kinase Leucine zipper bearing kinase Leucine zipper-bearing kinase LZK M3K13_HUMAN MAP3K13 MEKK13 MGC133196 Mitogen activated protein kinase kinase kinase 13 Mitogen-activated protein kinase kinase kinase 13 Mixed lineage kinase MLK OTTHUMP00000210397. |
| Product description | Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B. |
| Immunogen | KLH conjugated synthetic peptide derived from human MAP3K13/LZK 801-900/966 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseCowOrangutan |
| Concentration | 1mg/ml |
| Alternative Names | EC 2.7.11.25 Leucine zipper bearing kinase Leucine zipper bearing kinase Leucine zipper-bearing kinase LZK M3K13_HUMAN MAP3K13 MEKK13 MGC133196 Mitogen activated protein kinase kinase kinase 13 Mitogen-activated protein kinase kinase kinase 13 Mixed lineage kinase MLK OTTHUMP00000210397. |
|---|---|
| Molecular Weight(MW) | 108 KDa |
| Cellular Localization | Cytoplasm. Membrane. |
| SwissProt ID | O43283 |
|---|
Application
Fig1: Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-MAP3K13/LZK (ER1912-68) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MAP3K13) Polyclonal Antibody, Unconjugated (ER1912-68) at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.| Positive Control | Expressed in the adult brain, liver, placenta and pancreas, with expression strongest in the pancreas. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
