Application
Fig1: Sample: Lane1: Heart (Mouse) Lysate at 30 ug Lane2: Brain (Mouse) Cell Lysate at 30 ug Primary: Anti-MAP3K8/TPL2 (ER1912-69) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size: 53kD Observed band size: 50kDApplication
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-MAP3K8 (ER1912-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kDApplication
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-MAP3K8 (ER1912-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Product Name | MAP3K8 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | c COT Cancer Osaka thyroid oncogene Cancer Osaka thyroid oncogene CCOT COT COT proto oncogene serine/threonine protein kinase EST ESTF Ewing sarcoma transformant FLJ10486 M3K8_HUMAN MAP3K 8 MAP3K8 Mitogen activated protein kinase kinase kinase 8 Mitogen-activated protein kinase kinase kinase 8 Proto oncogene cCot Proto-oncogene c-Cot Serine/threonine protein kinase cot Serine/threonine-protein kinase cot TPL 2 TPL-2 TPL2 Tumor progression locus 2. |
| Product description | Required for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein. |
| Immunogen | KLH conjugated synthetic peptide derived from human MAP3K8/TPL2 121-220/468 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | c COT Cancer Osaka thyroid oncogene Cancer Osaka thyroid oncogene CCOT COT COT proto oncogene serine/threonine protein kinase EST ESTF Ewing sarcoma transformant FLJ10486 M3K8_HUMAN MAP3K 8 MAP3K8 Mitogen activated protein kinase kinase kinase 8 Mitogen-activated protein kinase kinase kinase 8 Proto oncogene cCot Proto-oncogene c-Cot Serine/threonine protein kinase cot Serine/threonine-protein kinase cot TPL 2 TPL-2 TPL2 Tumor progression locus 2. |
|---|---|
| Molecular Weight(MW) | 53 KDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P41279 |
|---|
Application
Fig1: Sample: Lane1: Heart (Mouse) Lysate at 30 ug Lane2: Brain (Mouse) Cell Lysate at 30 ug Primary: Anti-MAP3K8/TPL2 (ER1912-69) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution Predicted band size: 53kD Observed band size: 50kD
Application
Fig2: Sample: Thymus (Mouse) Lysate at 40 ug Primary: Anti-MAP3K8 (ER1912-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD
Application
Fig3: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti-MAP3K8 (ER1912-69) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53 kD Observed band size: 53 kD| Positive Control | Expressed in several normal tissues and human tumor-derived cell lines. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
