Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-MAP3K9 (ER1912-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 122 kD Observed band size: 122 kD| Product Name | MAP3K9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLK1 M3K9_HUMAN Map3k9 MEKK9 Mitogen activated protein kinase kinase kinase 9 Mitogen-activated protein kinase kinase kinase 9 Mixed lineage kinase 1 (tyr and ser/thr specificity) Mixed lineage kinase 1 PRKE1. |
| Product description | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. |
| Immunogen | KLH conjugated synthetic peptide derived from human MAP3K9 451-550/1104 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | MLK1 M3K9_HUMAN Map3k9 MEKK9 Mitogen activated protein kinase kinase kinase 9 Mitogen-activated protein kinase kinase kinase 9 Mixed lineage kinase 1 (tyr and ser/thr specificity) Mixed lineage kinase 1 PRKE1. |
|---|---|
| Molecular Weight(MW) | 122 KDa |
| SwissProt ID | 80192.0 |
|---|
Application
Fig1: Sample: MCF-7(Human) Cell Lysate at 30 ug Primary: Anti-MAP3K9 (ER1912-70) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 122 kD Observed band size: 122 kD| Positive Control | Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
