Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- MAPK4 (ER1912-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD| Product Name | MAPK4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERK-4 ERK3 Erk3 related ERK4 Extracellular signal regulated kinase 4 Extracellular signal-regulated kinase 4 MAP kinase 4 MAP kinase isoform p63 MAPK 4 MAPK-4 Mitogen activated protein kinase 4 Mitogen-activated protein kinase 4 MK04_HUMAN p63 MAPK p63-MAPK p63MAPK PRKM4. |
| Product description | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle (By similarity). |
| Immunogen | KLH conjugated synthetic peptide derived from human ERK5 401-500/587 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowHorseRabbit |
| Concentration | 1mg/ml |
| Alternative Names | ERK-4 ERK3 Erk3 related ERK4 Extracellular signal regulated kinase 4 Extracellular signal-regulated kinase 4 MAP kinase 4 MAP kinase isoform p63 MAPK 4 MAPK-4 Mitogen activated protein kinase 4 Mitogen-activated protein kinase 4 MK04_HUMAN p63 MAPK p63-MAPK p63MAPK PRKM4. |
|---|---|
| Molecular Weight(MW) | 66 KDa |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5. |
| SwissProt ID | P31152 |
|---|
Application
Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- MAPK4 (ER1912-73) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD| Positive Control | High expression in heart and brain. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
