Application
Fig1: Sample: Brain(Rat) lysate at 30ug; Primary: Anti-MASP1(ER1912-76) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 54/77kD Observed band size : 77kD| Product Name | MASP1/MASP3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MASP1/3 MASP-1 Complement factor MASP-3 Complement factor MASP3 Complement-activating component of Ra-reactive factor CRARF EC 3.4.21 Mannan binding lectin serine protease 1 Mannose binding lectin associated serine protease 1 Mannose binding protein associated serine protease Mannose-binding lectin-associated serine protease 1 Mannose-binding lectin-associated serine protease-1 MASP PRSS 5 Ra reactive factor serine protease p100 Ra-reactive factor serine protease p100 RaRF Serine protease 5 MASP1_HUMAN. Mannan-binding lectin serine protease 1 Mannan-binding lectin serine protease 1 heavy chain MASP1_HUMAN. |
| Product description | Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5. |
| Immunogen | KLH conjugated synthetic peptide derived from human MASP 20-120/699 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatDogPigCowRabbit |
| Concentration | 1mg/ml |
| Alternative Names | MASP1/3 MASP-1 Complement factor MASP-3 Complement factor MASP3 Complement-activating component of Ra-reactive factor CRARF EC 3.4.21 Mannan binding lectin serine protease 1 Mannose binding lectin associated serine protease 1 Mannose binding protein associated serine protease Mannose-binding lectin-associated serine protease 1 Mannose-binding lectin-associated serine protease-1 MASP PRSS 5 Ra reactive factor serine protease p100 Ra-reactive factor serine protease p100 RaRF Serine protease 5 MASP1_HUMAN. Mannan-binding lectin serine protease 1 Mannan-binding lectin serine protease 1 heavy chain MASP1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 49/77 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | P48740 |
|---|
Application
Fig1: Sample: Brain(Rat) lysate at 30ug; Primary: Anti-MASP1(ER1912-76) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 54/77kD Observed band size : 77kD| Positive Control | Protein of the plasma which is primarily expressed by liver. |
|---|---|
| Application Notes | WB:1:500-2000: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
