Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- MASP2 (ER1912-77) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 74 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (ER1912-77) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (ER1912-77) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig4: Sample: Plasma(Rat) Lysate at 40 ug Primary: Anti-MASP2 (ER1912-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 75 kD| Product Name | MASP2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | mannan-binding lectin serine peptidase 2 MAP19 MASP-2 Mannose-binding protein-associated serine protease 2 MBL-associated serine protease 2 MASP1P1 sMAP Small MBL associated protein.（Contains: Mannan-binding lectin serine protease 2 A chain Mannan-binding lectin serine protease 2 B chain）. |
| Product description | Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase. |
| Immunogen | KLH conjugated synthetic peptide derived from human MASP2 351-450/686 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatHorse |
| Concentration | 1mg/ml |
| Alternative Names | mannan-binding lectin serine peptidase 2 MAP19 MASP-2 Mannose-binding protein-associated serine protease 2 MBL-associated serine protease 2 MASP1P1 sMAP Small MBL associated protein.（Contains: Mannan-binding lectin serine protease 2 A chain Mannan-binding lectin serine protease 2 B chain）. |
|---|---|
| Molecular Weight(MW) | 74 KDa |
| Cellular Localization | Secreted. |
| SwissProt ID | O00187 |
|---|
Application
Fig1: Sample: Lung (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti- MASP2 (ER1912-77) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 74 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (ER1912-77) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MASP2) Polyclonal Antibody, Unconjugated (ER1912-77) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig4: Sample: Plasma(Rat) Lysate at 40 ug Primary: Anti-MASP2 (ER1912-77) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74 kD Observed band size: 75 kD| Positive Control | Plasma. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
