Application
Fig1: Tissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(ER1912-78) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig2: Sample: MDA-MB-231(Human) Cell Lysate at 40 ug Primary: Anti-Maspin (ER1912-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD| Product Name | Maspin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | mammary serine proteinase inhibitor Maspin Ovalbumin PI-5 PI5 Protease inhibitor 5 Serine (or cysteine) peptidase inhibitor, clade B, member 5 Serpin B5 serpin peptidase inhibitor Serpin peptidase inhibitor, clade B (ovalbumin), member 5 SERPINB5 SPB5_HUMAN Spi5 Spi7. |
| Product description | Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Immunogen | KLH conjugated synthetic peptide derived from human Maspin 301-375/375 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | mammary serine proteinase inhibitor
Maspin
Ovalbumin
PI-5
PI5
Protease inhibitor 5
Serine (or cysteine) peptidase inhibitor clade B member 5 Serpin B5 serpin peptidase inhibitor Serpin peptidase inhibitor clade B (ovalbumin) member 5 SERPINB5 SPB5_HUMAN Spi5 Spi7. |
|---|---|
| Molecular Weight(MW) | 42 KDa |
| Cellular Localization | Secreted, extracellular space. |
| SwissProt ID | P36952 |
|---|
Application
Fig1: Tissue/cell: Rat rectum tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Maspin Polyclonal Antibody, Unconjugated(ER1912-78) 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig2: Sample: MDA-MB-231(Human) Cell Lysate at 40 ug Primary: Anti-Maspin (ER1912-78) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD| Positive Control | Normal mammary epithelial cells. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
