Application
Fig1: Sample: stomach (mouse) Lysate at 40 ug Primary: Anti- MBOAT4(ER1912-81)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 55 kDApplication
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (ER1912-81) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Product Name | MBOAT4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FKSG89 ghrelin o transferas Ghrelin O-acyltransferase GOAT MBOA4_HUMAN Mboat4 Membrane-bound O-acyltransferase domain-containing protein 4 O-acyltransferase (membrane bound) domain containing 4 O-acyltransferase domain-containing protein 4 OACT4. |
| Product description | Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid. |
| Immunogen | KLH conjugated synthetic peptide derived from human Ghrelin O acyltransferase 101-200/435 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatDogHorseRabbitMonkeyGorilla |
| Concentration | 1mg/ml |
| Alternative Names | FKSG89 ghrelin o transferas Ghrelin O-acyltransferase GOAT MBOA4_HUMAN Mboat4 Membrane-bound O-acyltransferase domain-containing protein 4 O-acyltransferase (membrane bound) domain containing 4 O-acyltransferase domain-containing protein 4 OACT4. |
|---|---|
| Molecular Weight(MW) | 50 KDa |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein. |
| SwissProt ID | Q96T53 |
|---|
Application
Fig1: Sample: stomach (mouse) Lysate at 40 ug Primary: Anti- MBOAT4(ER1912-81)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50kD Observed band size: 55 kD
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MBOAT4) Polyclonal Antibody, Unconjugated (ER1912-81) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.| Positive Control | Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
