Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (ER1912-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kDApplication
Fig2: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (ER1912-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kDApplication
Fig3: Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(ER1912-83) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig4: Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(ER1912-83) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingApplication
Fig5: Blank control: 293T(blue). Primary Antibody: Rabbit Anti- MC1 Receptor/AF488 Conjugated antibody (ER1912-83-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1912-83-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.| Product Name | MC1 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MC1 Receptor CMM5 MC1-R MC-1R MC1R Melanocortin 1 receptor Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) Melanocortin receptor 1 Melanocyte-stimulating hormone receptor Melanotropin receptor MSH-R MSHR MSHR_HUMAN SHEP2. |
| Product description | Receptor for MSH (alpha, beta and gamma) and ACTH. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. |
| Immunogen | KLH conjugated synthetic peptide derived from mouse MC-1R 271-317/317 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-PFC |
| WB:1:500-2000: IHC-P:1:400-800: FC:1: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | MC1 Receptor CMM5 MC1-R MC-1R MC1R Melanocortin 1 receptor Melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor) Melanocortin receptor 1 Melanocyte-stimulating hormone receptor Melanotropin receptor MSH-R MSHR MSHR_HUMAN SHEP2. |
|---|---|
| Molecular Weight(MW) | 35 KDa |
| Cellular Localization | Cell membrane. |
| SwissProt ID | Q01727 |
|---|
Application
Fig1: Sample: Hela(Human) Cell Lysate at 30 ug Raji(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (ER1912-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD
Application
Fig2: Sample: MOLT-4(Human) Cell Lysate at 30 ug Primary: Anti-MC1 Receptor (ER1912-83) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 35 kD
Application
Fig3: Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(ER1912-83) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig4: Tissue/cell: human melanoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MSHR Polyclonal Antibody, Unconjugated(ER1912-83) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Application
Fig5: Blank control: 293T(blue). Primary Antibody: Rabbit Anti- MC1 Receptor/AF488 Conjugated antibody (ER1912-83-AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions. Protocol The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1912-83-AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.| Positive Control | Melanocytes and corticoadrenal tissue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: FC:1: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
