Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MCT4 Polyclonal Antibody, Unconjugated (ER1912-89) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MCT4) Polyclonal Antibody, Unconjugated (ER1912-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit (Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig3: Sample: HepG2 Cell Lysate at 30 ug Primary: Anti-MCT4 (ER1912-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kDApplication
Fig4: Sample: Muscle (Mouse) Lysate at 30 ug Primary: Anti-MCT4 (ER1912-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD| Product Name | MCT4/SLC16A3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Monocarboxylate transporter 4 Slc16a3 Mct3 Mct4 MCT-4 Monocarboxylate transporter 3 solute carrier family 16 member 3 SLC16A3 MOT4_HUMAN. |
| Product description | Proton-linked monocarboxylate transporter. Catalyzes the rapid transport across the plasma membrane of many monocarboxylates such as lactate, pyruvate, branched-chain oxo acids derived from leucine, valine and isoleucine, and the ketone bodies acetoacetate, beta-hydroxybutyrate and acetate. Lactic acid and pyruvate transport across plasma membranes is catalyzed by members of the proton-linked monocarboxylate transporter (MCT) family, which has been designated solute carrier family-16. Each MCT appears to have slightly different substrate and inhibitor specificities and transport kinetics, which are related to the metabolic requirements of the tissues in which it is found. The MCTs, which include MCT1 and MCT2, are characterized by 12 predicted transmembrane domains |
| Immunogen | KLH conjugated synthetic peptide derived from human SLC16A3 401-465/465 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatChickenDogCowHorse |
| Concentration | 1mg/ml |
| Alternative Names | Monocarboxylate transporter 4 Slc16a3 Mct3 Mct4 MCT-4 Monocarboxylate transporter 3 solute carrier family 16 member 3 SLC16A3 MOT4_HUMAN. |
|---|---|
| Molecular Weight(MW) | 52 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | O15427 |
|---|
Application
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MCT4 Polyclonal Antibody, Unconjugated (ER1912-89) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining.
Application
Fig2: Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MCT4) Polyclonal Antibody, Unconjugated (ER1912-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit (Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig3: Sample: HepG2 Cell Lysate at 30 ug Primary: Anti-MCT4 (ER1912-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD
Application
Fig4: Sample: Muscle (Mouse) Lysate at 30 ug Primary: Anti-MCT4 (ER1912-89) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 52kD Observed band size: 52kD| Positive Control | Highly expressed in skeletal muscle. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
