Application
Fig1: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (ER1912-91),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.Application
Fig2: Sample: Liver (mouse) Lysate at 40 ug Primary: Anti- MDR1 (ER1912-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 141kD Observed band size: 150kD| Product Name | MDR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P-Glycoprotein Multi Drug Resistance Associated Protein, ABCB1 ATP-binding cassette sub-family B (MDR/TAP), member 1 ABC20 CD243 CD243 antigen CLCS Multidrug resistance protein 1 P glycoprotein 1 p-GP gp170 P gp PGY1 MDR1_HUMAN. |
| Product description | Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells. |
| Immunogen | KLH conjugated synthetic peptide derived from human MDR1 21-100/1272 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:1μg/Test: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | P-Glycoprotein
Multi Drug Resistance Associated Protein ABCB1 ATP-binding cassette sub-family B (MDR/TAP) member 1 ABC20 CD243 CD243 antigen CLCS Multidrug resistance protein 1 P glycoprotein 1 p-GP gp170 P gp PGY1 MDR1_HUMAN. |
|---|---|
| Molecular Weight(MW) | 141 KDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
| SwissProt ID | P21447 |
|---|
Application
Fig1: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-MDR1 antibody (ER1912-91),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Application
Fig2: Sample: Liver (mouse) Lysate at 40 ug Primary: Anti- MDR1 (ER1912-91)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 141kD Observed band size: 150kD| Positive Control | Expressed in liver, kidney, small intestine and brain. |
|---|---|
| Application Notes | WB:1:500-2000: FC:1μg/Test: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
