Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MED12) Polyclonal Antibody, Unconjugated (ER1912-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- MED12/Trap230 (ER1912-92) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 243kD Observed band size: 243kD| Product Name | MED12/Trap230 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component ARC240 CAG repeat protein 45 CAGH45 HOPA KIAA0192 MED12 MED12_HUMAN Mediator complex subunit 12 Mediator of RNA polymerase II transcription subunit 12 Mediator of RNA polymerase II transcription subunit 12 Mediator of RNA polymerase II transcription subunit 12 OPA containing protein OPA-containing protein OPA-containing protein Thyroid hormone receptor associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component TNRC11 Trap230 Trinucleotide repeat containing gene 11 protein Trinucleotide repeat-containing gene 11 protein Trinucleotide repeat-containing gene 11 protein. |
| Product description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. |
| Immunogen | KLH conjugated synthetic peptide derived from human MED12/Trap230 251-350/2177 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRatPigCowRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Activator recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component Activator-recruited cofactor 240 kDa component ARC240 CAG repeat protein 45 CAGH45 HOPA KIAA0192 MED12 MED12_HUMAN Mediator complex subunit 12 Mediator of RNA polymerase II transcription subunit 12 Mediator of RNA polymerase II transcription subunit 12 Mediator of RNA polymerase II transcription subunit 12 OPA containing protein OPA-containing protein OPA-containing protein Thyroid hormone receptor associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component Thyroid hormone receptor-associated protein complex 230 kDa component TNRC11 Trap230 Trinucleotide repeat containing gene 11 protein Trinucleotide repeat-containing gene 11 protein Trinucleotide repeat-containing gene 11 protein. |
|---|---|
| Molecular Weight(MW) | 243 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q93074 |
|---|
Application
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (MED12) Polyclonal Antibody, Unconjugated (ER1912-92) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Application
Fig2: Sample: Spleen (Mouse) Lysate at 40 ug Primary: Anti- MED12/Trap230 (ER1912-92) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 243kD Observed band size: 243kD| Positive Control | Ubiquitous. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
