Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti- MED4 (ER1912-93) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 30kD| Product Name | MED4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activator-recruited cofactor 36 kDa component ARC36 DRIP36 FLJ10956 HSPC126 med4 MED4_HUMAN Mediator complex subunit 4 Mediator of RNA polymerase II transcription subunit 4 Mediator of RNA polymerase II transcription, subunit 4 homolog OTTHUMP00000018390 OTTHUMP00000018391 OTTHUMP00000018392 RP11-90M2.2 TRAP/SMCC/PC2 subunit p36 subunit TRAP36 VDRIP Vitamin D3 receptor-interacting protein complex 36 kDa component. |
| Product description | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Immunogen | KLH conjugated synthetic peptide derived from human MED4 101-200/270 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-2000: |
|
| Species Reactivity | HumanMouseRatChickenDogPigCowHorseRabbitSheep |
| Concentration | 1mg/ml |
| Alternative Names | Activator-recruited cofactor 36 kDa component
ARC36
DRIP36
FLJ10956
HSPC126
med4
MED4_HUMAN
Mediator complex subunit 4
Mediator of RNA polymerase II transcription subunit 4
Mediator of RNA polymerase II transcription subunit 4 homolog OTTHUMP00000018390 OTTHUMP00000018391 OTTHUMP00000018392 RP11-90M2.2 TRAP/SMCC/PC2 subunit p36 subunit TRAP36 VDRIP Vitamin D3 receptor-interacting protein complex 36 kDa component. |
|---|---|
| Molecular Weight(MW) | 30 KDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | Q9NPJ6 |
|---|
Application
Fig1: Sample: A549 Cell (Human) Lysate at 30 ug Primary: Anti- MED4 (ER1912-93) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30kD Observed band size: 30kD| Application Notes | WB:1:500-2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
