Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-MHC class I antibody (ER1912-99) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Application
Fig2: Sample: HL-60(Human) Cell Lysate at 30 ug Primary: Anti-MHC class I (ER1912-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 42 kD| Product Name | MHC class I |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MHC-1 MHC1 MHC 1 A 28 A 9 Aw 24 Aw 68 CLASS I HISTOCOMPATIBILITY ANTIGEN H2 K1 H2K HLA A HLAA HLA-A HLA class I histocompatibility antigen A 1 alpha chain HLA class I histocompatibility antigen A 11 alpha chain HLA class I histocompatibility antigen A 2 alpha chain HLA class I histocompatibility antigen A 24 alpha chain HLA class I histocompatibility antigen A 3 alpha chain HLA class I histocompatibility antigen A 30 alpha chain HLA class I histocompatibility antigen A 32 alpha chain HLA class I histocompatibility antigen A 68 alpha chain Major histocompatibility complex class I A MHC class I heavy chain H2 K 1A24_HUMAN. |
| Product description | Involved in the presentation of foreign antigens to the immune system. |
| Immunogen | KLH conjugated synthetic peptide derived from human HLA-A 51-150/365 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500-2000: FC:3ug/Test: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MHC-1 MHC1 MHC 1 A 28 A 9 Aw 24 Aw 68 CLASS I HISTOCOMPATIBILITY ANTIGEN H2 K1 H2K HLA A HLAA HLA-A HLA class I histocompatibility antigen A 1 alpha chain HLA class I histocompatibility antigen A 11 alpha chain HLA class I histocompatibility antigen A 2 alpha chain HLA class I histocompatibility antigen A 24 alpha chain HLA class I histocompatibility antigen A 3 alpha chain HLA class I histocompatibility antigen A 30 alpha chain HLA class I histocompatibility antigen A 32 alpha chain HLA class I histocompatibility antigen A 68 alpha chain Major histocompatibility complex class I A MHC class I heavy chain H2 K 1A24_HUMAN. |
|---|---|
| Molecular Weight(MW) | 40 KDa |
| Cellular Localization | Cell Membrane; Type I membrane protein. |
| SwissProt ID | P05534 |
|---|
Application
Fig1: Blank control: A431. Primary Antibody (green line): Rabbit Anti-MHC class I antibody (ER1912-99) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody: Goat anti-rabbit IgG-AF647 Dilution: 3μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Application
Fig2: Sample: HL-60(Human) Cell Lysate at 30 ug Primary: Anti-MHC class I (ER1912-99) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 40 kD Observed band size: 42 kD| Application Notes | WB:1:500-2000: FC:3ug/Test: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
