Application
Fig1: Protein: mouse lung lysates at 30ug; Primary: Anti-MHC Class II (ER1913-01) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 27kD Observed band size : 30kDApplication
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated(ER1913-01) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Product Name | MHC Class II |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DMA DMB DRB HLA class II histocompatibility antigen DM beta chain HLA DMB HLADM HLADP1B Major histocompatibility complex clas HLA class II histocompatibility antigen, DM beta chain MHC class II antigen DMB Really interesting new gene 7 protein HLA-DMB RING7 DMB_HUMAN MHC2. |
| Product description | Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. |
| Immunogen | KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain 111-200/263 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-P |
| WB:1:500-2000: IHC-P:1:400-800: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | DMA
DMB
DRB HLA class II histocompatibility antigen DM beta chain HLA DMB HLADM HLADP1B Major histocompatibility complex clas HLA class II histocompatibility antigen DM beta chain MHC class II antigen DMB Really interesting new gene 7 protein HLA-DMB RING7 DMB_HUMAN MHC2. |
|---|---|
| Molecular Weight(MW) | 27 KDa |
| Cellular Localization | Cell membrane. Endoplasmic reticulum membrane. |
| SwissProt ID | P28068 |
|---|
Application
Fig1: Protein: mouse lung lysates at 30ug; Primary: Anti-MHC Class II (ER1913-01) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 27kD Observed band size : 30kD
Application
Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MHC Class II Polyclonal Antibody, Unconjugated(ER1913-01) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining| Positive Control | Mouse lung lysates, human lung carcinoma issue. |
|---|---|
| Application Notes | WB:1:500-2000: IHC-P:1:400-800: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
